Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS.

By |October 30th, 2009|Publicacions|0 Comments|

Rodríguez-Medina IC, Beltrán-Debón R, Molina VM, Alonso-Villaverde C, Joven J, Menéndez JA, Segura-Carretero A, Fernández-Gutiérrez A.

Source
Faculty of Sciences, Department of Analytical Chemistry, University of Granada, Granada, Spain.

Abstract

The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization […]