Monthly Archives: September 2015

23 09, 2015
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    Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice

Paraoxonase-1 Deficiency does not Influence Clopidogrel Antiplatelet Function in Mice

By |September 23rd, 2015|Publicacions|0 Comments|

Anabel García-Heredia1, Anna Hérnandez-Aguilera1, Isabel Fort-Gallifa1, Jorge Joven1, Vicente Martín-Paredero2 and Jordi Camps1*

1Unitat de Recerca Biomèdica (AGH, AHA, IFG, JJ, JC), Hospital Universitari de Sant Joan
2Service of Angiology, Vascular Surgery and Endosurgery, Hospital Universitari Joan XXIII (VMP), Institut d’Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, Spain

Abstract
Background: Clopidogrel is an antiplatelet agent used in the treatment of vascular diseases. It requires in vivo bio activation linked to the cytochrome P450. Several studies reported that paraoxonase-1 (PON1) was a crucial enzyme in clopidogrel activation, and that patients carrying a variant of the PON1192 gene polymorphism have a high risk of thrombosis. However, these reports were not confirmed by subsequent results. The present study was aimed at investigating whether PON1 […]

23 09, 2015

Visceral Artery Aneurysms: A Complex and Unresolved Issue

By |September 23rd, 2015|Publicacions|0 Comments|

Arefai B1, Perea-Ribis M2, Gómez B1, Rodríguez N1, Hernández P3, Camps J2, Joven J2, Hernández-Aguilera A2*, Martín-Paredero V1.

1Servei d’Angiologia, Cirurgia Vascular i Endovascular, Hospital Universitari Joan XXIII, Tarragona, Spain.
2 Unitat de Recerca Biomèdica, Universitat Rovira i Virgili, Reus, Tarragona, Spain.
3 Servei de Radiologia i Medicina Nuclear, Hospital Universitari Joan XXIII, Tarragona, Spain.

ABSTRACT

Aims: The lack of prospective studies on abdominal non-aortic true or false aneurysms results in insufficient data to predict their natural history and propose a treatment of choice. The experience provided by case reports is important to discuss their management and prevention. This is relevant because abnormal enlargements in superior mesenteric artery are relatively uncommon but associated with specific symptoms and a high incidence of rupture and/or ischemic […]

23 09, 2015
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    Exploring the Process of Energy Generation in Pathophysiology by Targeted Metabolomics: Performance of a Simple and Quantitative Method

Exploring the Process of Energy Generation in Pathophysiology by Targeted Metabolomics: Performance of a Simple and Quantitative Method

By |September 23rd, 2015|Publicacions|0 Comments|

Riera-Borrull M1,2, Rodríguez-Gallego E1,2, Hernández-Aguilera A1,2, Luciano F1,2, Ras R3, Cuyàs E4,5, Camps J1,2, Segura-Carretero A6,7, Menendez JA4,5,Joven J1,2, Fernández-Arroyo S1,2.

1Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, IISPV, Universitat Rovira i Virgili, Reus, Spain.
2Campus of International Excellence Southern Catalonia, Tarragona, Spain.
3Center for Omics Sciences, Reus, Spain.
4Metabolism and Cancer Group, Translational Research Laboratory, Catalan Institute of Oncology (ICO), Girona, Spain.
5Girona Biomedical Research Institute (IDIBGI), Girona, Spain.
6Department of Analytical Chemistry, University of Granada, Granada, Spain.
7Research and Development of Functional Food Centre (CIDAF), Granada, Spain.

Abstract

Abnormalities in mitochondrial metabolism and regulation of energy balance contribute to human diseases. The consequences of high fat and other nutrient intake, and the resulting acquired mitochondrial dysfunction, are essential to fully understand common disorders, including […]

23 09, 2015
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    The acute impact of polyphenols from Hibiscus sabdariffa in metabolic homeostasis: an approach combining metabolomics and gene-expression analyses

The acute impact of polyphenols from Hibiscus sabdariffa in metabolic homeostasis: an approach combining metabolomics and gene-expression analyses

By |September 23rd, 2015|Publicacions|0 Comments|

Beltrán-Debón R1, Rodríguez-Gallego E, Fernández-Arroyo S, Senan-Campos O, Massucci FA, Hernández-Aguilera A, Sales-Pardo M, Guimerà R, Camps J,Menendez JA, Joven J.

Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Campus of International excellence Southern Catalonia, Carrer Sant Llorenç 21, 43201-Reus, Spain.
Abstract

We explored the acute multifunctional effects of polyphenols from Hibiscus sabdariffa in humans to assess possible consequences on the host’s health. The expected dynamic response was studied using a combination of transcriptomics and metabolomics to integrate specific functional pathways through network-based methods and to generate hypotheses established by acute metabolic effects and/or modifications in the expression of relevant genes. Data were obtained from healthy male volunteers after 3 hours of ingestion […]

23 09, 2015
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    Reshaping of Human Macrophage Polarization through Modulation of Glucose Catabolic Pathways.

Reshaping of Human Macrophage Polarization through Modulation of Glucose Catabolic Pathways.

By |September 23rd, 2015|Publicacions|0 Comments|

Izquierdo E1, Cuevas VD1, Fernández-Arroyo S2, Riera-Borrull M2, Orta-Zavalza E1, Joven J2, Rial E1, Corbi AL1, Escribese MM3.

1Centro de Investigaciones Biológicas, 28040 Madrid, Spain;
2Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, 43201 Reus, Spain; and Campus of International Excellence Southern Catalonia, 43201 Reus, Spain.
3Centro de Investigaciones Biológicas, 28040 Madrid, Spain

Abstract

Macrophages integrate information from the tissue microenvironment and adjust their effector functions according to the prevalent extracellular stimuli. Therefore, macrophages can acquire a variety of activation (polarization) states, and this functional plasticity allows the adequate initiation, regulation, and resolution of inflammatory responses. Modulation of the glucose metabolism contributes to the macrophage adaptation to the surrounding cytokine milieu, as exemplified by the […]

23 09, 2015
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    Liver fat deposition and mitochondrial dysfunction in morbid obesity: An approach combining metabolomics with liver imaging and histology

Liver fat deposition and mitochondrial dysfunction in morbid obesity: An approach combining metabolomics with liver imaging and histology

By |September 23rd, 2015|Publicacions|0 Comments|

Calvo N, Beltrán-Debón R, Rodríguez-Gallego E, Hernández-Aguilera A, Guirro M, Mariné-Casadó R, Millá L, Alegret JM, Sabench F, del Castillo D,Vinaixa M, Rodríguez MÀ, Correig X, García-Álvarez R, Menendez JA, Camps J, Joven J.

Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Campus of International Excellence Southern Catalonia, 43201 Reus, Spain

Abstract

AIM:
To explore the usefulness of magnetic resonance imaging (MRI) and spectroscopy (MRS) for assessment of non-alcoholic fat liver disease (NAFLD) as compared with liver histological and metabolomics findings.
METHODS:
Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesity-induced NAFLD in an observational, prospective, single-site, cross-sectional study with a pre-set duration of 1 year. […]

23 09, 2015
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    Lemon verbena (Lippia citriodora) polyphenols alleviate obesity-related disturbances in hypertrophic adipocytes through AMPK-dependent mechanisms

Lemon verbena (Lippia citriodora) polyphenols alleviate obesity-related disturbances in hypertrophic adipocytes through AMPK-dependent mechanisms

By |September 23rd, 2015|Publicacions|0 Comments|

Herranz-López M1, Barrajón-Catalán E1, Segura-Carretero A2, Menéndez JA3, Joven J4, Micol V5.

1Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain.
2Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
3Metabolism & Cancer Group, Translational Research Laboratory, Catalan Institute of Oncology and Biomedical Research Institute, Girona, Spain.
4Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, IISPV, Universitat Rovira i Virgili, C/ Sant Joan s/n, 43201 Reus, Spain.
5Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain; CIBER (CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III), Spain.

Abstract

BACKGROUND:
There is growing evidence that natural products, mostly plant-derived polyphenols, are important in the relationship between nutrients and health in humans.
PURPOSE:
We […]

23 09, 2015
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    Immunohistochemical analysis of paraoxonases and chemokines in arteries of patients with peripheral artery disease.

Immunohistochemical analysis of paraoxonases and chemokines in arteries of patients with peripheral artery disease.

By |September 23rd, 2015|Publicacions|0 Comments|

Hernández-Aguilera A1, Sepúlveda J2, Rodríguez-Gallego E1, Guirro M1, García-Heredia A1, Cabré N1, Luciano-Mateo F1, Fort-Gallifa I1, Martín-Paredero V2,Joven J1, Camps J1.

1Biomedical Research Unit, Hospital Universitari de Sant Joan, Institut d’Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Reus, Catalonia 43201, Spain. [email protected]
2Service of Angiology, Vascular Surgery and Endosurgery, Hospital Universitari Joan XXIII, Institut d’Investigacio Sanitaria Pere Virgili, Universitat Rovira i Virgili, Tarragona, Catalonia 43005, Spain.

Abstract

Oxidative damage to lipids and lipoproteins is implicated in the development of atherosclerotic vascular diseases, including peripheral artery disease (PAD). The paraoxonases (PON) are a group of antioxidant enzymes, termed PON1, PON2, and PON3 that protect lipoproteins and cells from peroxidation and, as such, may be involved in protection against the atherosclerosis process. PON1 inhibits […]